OMIP‐057: Mouse γδ T‐Cell Development Characterized by a 14 Color Flow Cytometry Panel

Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population.

We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lym...

Diagnostic Application of Two-Color Flow Cytometry

Recent immunophenotypic studies of hairy cell leukemia (HCL) have suggested specific patterns of immunoreactivity that may aid in diagnosis. We studied peripheral blood (PB) from 1 61 cases of HCL using two-color direct immunofluorescence flow cytometry and an extended panel of antibody combinations. Circulating hairy cells were identified by immunophenotypic features in 92% of the cases and co...

Studying Mouse Thymocyte Development using Multiparametric Flow Cytometry: An Efficient Method to Improve an 8-Color Panel on the BD FACSVerseTM System Application Note

OMIP-020: phenotypic characterization of human γδ T-cells by multicolor flow cytometry.

THIS panel was composed and optimized to investigate the differentiation stages of human cd T-cells in cryopreserved peripheral blood mononuclear cells (PBMC) from healthy individuals (Tables 1 and 2). As the majority of pan-cd T-cell antibodies available commercially proved to be inappropriate for detecting all cd T-cell populations in combination with other markers, this panel provides an ess...

Evaluation of Bt (Bacillus thuringiensis) corn on mouse testicular development by dual parameter flow cytometry.

The health safety of Bt (Bacillus thuringiensis) corn (Zea mays L.) was studied using mouse testes as a sensitive biomonitor of potential toxic effects. Pregnant mice were fed a Bt corn or a nontransgenic (conventional) diet during gestation and lactation. After they were weaned, young male mice were maintained on the respective diets. At 8, 16, 26, 32, 63, and 87 days after birth, three male m...